Legend: Example Technology:
Introduction of the gene encoding insulin in bacterial plasmids that
in turn carries the gene replication in bacterial cells, leading man
insulin. Plasmids: Plasmids are small circles of DNA found in cells of bacteria
separate from the bacterial chromosome and smaller than he. They are able to take
easily from one cell to another, even when the cells are clearly
different species, far apart on the evolutionary scale. So
plasmids can be used as vectors
,
playback allows foreign DNA by a bacterial replication system. cDNA human: genes consist of coding sequences and nekoduyuchyh. Copies of the coding sequences is called cDNA. It can be obtained from
reverse transcription of RNA. Transcription and translation of insulin cDNA will >> << production of functional molecules of insulin. Transfer of insulin gene Plasmid vector. Plasmids include both threads on, leaving a free, sticky end which DNA can be attached. Special communication sequences are added to human cDNA that he
fit exactly in the free ends opened plasmid DNA ring. Plasmids containing the human gene, also called recombinant plasmids,
ready to be inserted into another organism, such as bacterial cell >>. Recombinant plasmids << and bacterial cells are mixed. Plasmids
introduces bacteria in a process called transfection
. With the recombinant DNA molecule
successfully inserted into the bacterial host, the other object >> << plasmids can be used - their ability to multiply. After oral administration >> << bacteria, plasmids containing cDNA people can multiply to give
few dozen copies. When strattera 25mg bacteria divide, plasmids share
between the two daughter cells and plasmids continue to reproduce. Since
cell division rapidly (every 20 minutes), bacteria containing human
cDNA (coding for insulin, for example), will soon produce many millions
similar cells (clones) containing the same human gene. From: BIO. Biology in the future. Washington, DC: Biotechnology Industry Organization >> << 1990. .
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